A Potential New Isolate for Actinokinase Production
نویسندگان
چکیده
The study aimed to produce a potent fibrinolytic enzyme actinokinase from local isolate in batch fermentation, culture of identified thermophilic Streptomyces spp, grown on glucose yeast extract peptone medium pH of 8.0. The haemolytic activity of the crude enzyme and time spend for complete lysis was tested using blood agar media, and test tubes containing clotted blood. The in-vivo clot lyses of the crude enzyme was found to be faster (20 min) compared to the other commercial fibrinolytic enzyme (90 min). The enzyme was stable at a broad range of pH ranging from 5 to 9. This particular isolate could produce a promising actinokinase with thrombolytic potent activity.
منابع مشابه
Cloning and expression of an actinokinase gene from a thermophilic Streptomyces in Escherechia coli.
A thermophilic Streptomyces megasporus strain SD5, could secrete a new fibrinolytic (actinokinase) at 55 degrees C. The gene (ackS) encoding actinokinase was isolated from the chromosomal DNA of S. megasporus SD5 and cloned in different hosts and vectors. The expression was obtained in E. coli JM109 using Cla I linearized pBR322 as vector (pSR 500). The recombinant E. coli containing pSR 500 ex...
متن کاملAntimicrobial Activity of Indigenous Strains of Aureobasidium Isolated From Santalum Album Leaves
Although more than 3000 antibiotics have been reported from Actinomycetes/ higher fungi; biotechnological potential of yeasts and yeast-like fungi with respect to production of antimicrobial compounds has not been sufficiently investigated. We examined the antimicrobial activity of 11 strains of Aureobasidium pullulans (two new isolates and nine standard strains). All the strains of Aureobasidi...
متن کاملAntimicrobial Activity of Indigenous Strains of Aureobasidium Isolated From Santalum Album Leaves
Although more than 3000 antibiotics have been reported from Actinomycetes/ higher fungi; biotechnological potential of yeasts and yeast-like fungi with respect to production of antimicrobial compounds has not been sufficiently investigated. We examined the antimicrobial activity of 11 strains of Aureobasidium pullulans (two new isolates and nine standard strains). All the strains of Aureobasidi...
متن کاملBiosynthesis of gold nanoparticles using streptomyces fulvissimus isolate
Objective(s): In recent years, the biosynthesis of gold nanoparticles has been the focus of interest because of their emerging application in a number of areas such as biomedicine. In the present study we report the extracellular biosynthesis of gold nanoparticles (AuNPs) by using a positive bacterium named Streptomyces fulvissimus isolate U from rice fields of Guilan Province, Iran.Materials a...
متن کاملInvestigation of Acid-Neutralizing Property of Bacillus cereus GUF8
Extreme pHs, especially acidic pHs, are among the environmental stresses that some bacteria face. The most important strategies that bacteria employ for acid adaptation include either production of acidophilic proteins, or raising the pH of the microenvironment by secretion of basic compounds including ammonia produced by the action of urease. Considering the importance of acid-neutralizing bac...
متن کامل